Platform for Screening Abiotic/Biotic Interactions Using Indicator Displacement Assays

Indicator displacement assays that detect bilayer membranes enriched in phosphatidylserine

Three indicator displacement assays are described for the detection of phosphatidylserine in a bilayer membrane. A series of Zn-dipicolylamine coordination compounds are used to bind selectively to the phosphatidylserine and act as a colorimetric chemosensing ensemble when combined with the UV-Vis indictor pyrocatechol violet. A similar displacement assay uses a coumarin methylsulfonate derivat...

Using ratiometric indicator-displacement assays in semi-quantitative colorimetric determination of chloride, bromide, and iodide anions.

A ratiometric indicator-displacement assay (RIDA) array has been developed for the semi-quantitative colorimetric determination of chloride, bromide, and iodide anions. Determinations of these halide anions follow the displacement reaction using the chelate compound of (2-(3,5-dibromo-2-pyridylazo)-5-(diethylamino)phenol) (3,5-Br2-PADAP) and heavy metal salts as colorimetric reagent. Different ...

Transporter assays using solid supported membranes: a novel screening platform for drug discovery.

Transporters are important targets in drug discovery. However, high throughput-capable assays for this class of membrane proteins are still missing. Here we present a novel drug discovery platform technology based on solid supported membranes. The functional principles of the technology are described, and a sample selection of transporter assays is discussed: the H(+)-dependent peptide transpor...

Are two better than one? Comparing intermolecular and intramolecular indicator displacement assays in pyrophosphate sensors.

Peptide receptors with Zn(ii)-DPA units and a covalently bound fluorescent coumarin indicator on an oxazole-containing scaffold are shown to function as more selective pyrophosphate sensors than the analogous chemosensing ensembles in indicator displacement assays.

Application of Small Molecule Receptors to the Analysis of Post- Translational Modifications Using Fluorescent Indicator Displacement Assays